Preparation of RNA from cultured adherent and suspension cells for RT-PCR
The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents. The QuickExtract RNA Extraction Solution works with cultured adherent and suspension cells including buccal cells, and has been tested on human, mouse, rat, E. coli, and S. aureus cell cultures. It is not suitable for tissue samples or plant samples. An optional DNase I treatment may improve certain downstream applications.
Fast: Extract end-point and real-time RT-PCR ready RNA in minutes from low to high numbers of samples
Simple: Single tube protocol
Compatible: Extracted RNA is compatible with both real-time and endpoint RT-PCR
Automation-friendly: Simple protocol makes incorporation into an automated workflow easy
Flexible: A Dnase I digestion step can be added for sensitive downstream applications when necessary
Safe: No toxic reagents used
Storage:
Store the kit at –20°C in a freezer without a defrost cycle
Storage Buffer:
RNase-Free DNase I is supplied in a 50% glycerol solution containing 10 mM Tris-HCl (pH 7.5), 10 mM CaCl2, and 10 mM MgCl2
Unit Definition:
One Molecular Biology Unit (MBU) of RNase-Free DNase I converts 1 μg of pUC19 DNA into oligodeoxynucleotides in 10 minutes at 37°C
Quality Control:
The QuickExtract RNA Extraction Kit is function-tested by assaying for a PCR product from cDNA produced from RNA extracted from HeLa cells
Contaminating Activity Assays:
All components of the QuickExtract RNA Extraction Kit are free of detectable RNase activity, and all of the components except DNase I are free of detectable exo- and endonuclease activities
