Rapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples
The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).
The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.
Fast: PCR-ready DNA in about 8 minutes for most samples
Simple: No bead-beating, freezing, or grinding of plant leaf material
Increased Yields: No centrifugation or spin columns used which reduce yields
Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
Safe: Nontoxic reagets used throughout the procedure
Storage:
Store at -20 °C in a freezer without a defrost cycle. Minimise the number of freeze/thaw cycles. Thawed QuickExtract solution can be stored at 4 °C for 1 month or refrozen in small aliquots.
Quality control:
QuickExtract Plant DNA Extraction Solution is functionally-tested by assaying for the production of a PCR product from spinach leaf. A single-copy gene for heat shock protein HSP70 is used as the PCR template.
