MMLV REVERSE TRANSCRIPTASE 1ST-STRAND CDNA SYNTHESIS KIT 50 rxns -20º C

• Synthesize full-length cDNA from RNA templates longer than 15 kb
• MMLV HP RT demonstrates significantly higher activity than competitive reverse transcriptase enzymes
• Reaction Buffer, optimized for producing full-length cDNA, is included with both the MMLV HP RT and the MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit
• The kit includes both an oligo(dT) and random nonamer primers
• First-strand cDNA can be made from picogram amounts of input total RNA
• The kit includes a potent RNase Inhibitor to protect the integrity of template RNA

Applications

• First-strand cDNA synthesis for subsequent PCR or real-time PCR
• RT-PCR validation of gene expression data obtained from microarray experiments
• RT-PCR validation and quantification of gene silencing by RNA interference