You wouldn’t start a car that has been stationary for months without a maintenance check. Don’t start waking up those cells without completing these steps!

 
 
  • Make sure to turn off the power to your incubator and the humidity pan is empty
  • Take the time to complete a full interior wipe down with 70% EtOH
Run the appropriate decontamination cycle for your incubator:
  • Vapourized Hydrogen Peroxide Cycle
  • 180°C High Heat Sterilization Cycle
  • 24 hour UV Cycle
  1. Run the incubator at 37°C for 24 hours without gas
  2. Check the gas levels of your CO2 or N2 tanks. Replace as needed
  3. That gas won't go far if your tubes are frayed or cracked — so inspect and replace. And don't forget your inline filters too!
  4. You are now ready to open the gas supply
  5. Run a gas calibration — or better yet, contact a service professional to complete the procedure to ensure calibrated gas levels