This assay includes analytes that represent the following cardiac drug classes: amiodarone (antiarrhythmic agent), digoxin (digitalis), digitoxin (digitalis), verapamil (Ca+ channel blocker), furosemide (diuretic), and flecainide (antiarrhythmic agent).
Peaks tR (min) Precursor Ion Product Ion Product Ion Polarity
1. Digoxin 0.95 798.4 651.4 355.1 +
2. Flecainide 1.28 415.3 397.9 300.9 +
3. Furosemide 1.32 329.0 205.2 285.1 -
4. Verapamil 1.68 455.1 165.2 150.0 +
5. Digitoxin 1.85 782.4 635.2 243.1 +
6. Amiodarone 3.17 646.1 58.1 86.2 +
Restek_Force_cardiac
LC_CF0675
Column Force Biphenyl (cat.# 9629252)
Dimensions: 50 mm x 2.1 mm ID
Particle Size: 1.8 µm
Temp.: 40 °C
Sample
Diluent: Water:acetonitrile (90:10)
Conc.: 100 ng/mL
Inj. Vol.: 2 µL
Mobile Phase
A: Water + 5 mM ammonium formate + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
Time (min) Flow (mL/min) %A %B
0.00 0.3 60 40
3.50 0.3 5 95
3.51 0.3 60 40
5.5 0.3 60 40
Detector MS/MS
Ion Mode: ESI+/ESI-
Mode: Scheduled MRM
Instrument UHPLC
--> Many cardiac drugs must be closely monitored by clinical labs to ensure that the levels in patients remain within the drug’s therapeutic range, which can be quite narrow. A 1.8 µm fully porous particle Force Biphenyl column, along with detection by mass spectrometry, renders a fast and sensitive UHPLC assay in just 5.5 minutes. Polarity switching is used to allow the simultaneous analysis of acidic and basic compounds.
View Mandel's selection of Restek Force columns.
This assay includes analytes that represent the following cardiac drug classes: amiodarone (antiarrhythmic agent), digoxin (digitalis), digitoxin (digitalis), verapamil (Ca+ channel blocker), furosemide (diuretic), and flecainide (antiarrhythmic agent).
Peaks tR (min) Precursor Ion Product Ion Product Ion Polarity
1. Digoxin 0.95 798.4 651.4 355.1 +
2. Flecainide 1.28 415.3 397.9 300.9 +
3. Furosemide 1.32 329.0 205.2 285.1 -
4. Verapamil 1.68 455.1 165.2 150.0 +
5. Digitoxin 1.85 782.4 635.2 243.1 +
6. Amiodarone 3.17 646.1 58.1 86.2 +
Restek_Force_cardiac
LC_CF0675
Column Force Biphenyl (cat.# 9629252)
Dimensions: 50 mm x 2.1 mm ID
Particle Size: 1.8 µm
Temp.: 40 °C
Sample
Diluent: Water:acetonitrile (90:10)
Conc.: 100 ng/mL
Inj. Vol.: 2 µL
Mobile Phase
A: Water + 5 mM ammonium formate + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid
Time (min) Flow (mL/min) %A %B
0.00 0.3 60 40
3.50 0.3 5 95
3.51 0.3 60 40
5.5 0.3 60 40
Detector MS/MS
Ion Mode: ESI+/ESI-
Mode: Scheduled MRM
Instrument UHPLC